DECAPSULATION PROCEEDURE

FOR BRINE SHRIMP EGGS

(ARTEMIA CYSTS)

• A cone shaped or conical bottomed vessel should be used as the hatching container. This is used to maintain the cysts in suspension when aeration is applied at the bottom and to avoid any “dead spots”. Hatching water should have a salinity of 20ppt or specific gravity of 1,010 to 1,020
• Hatching water should have a pH in the range of 8.0 to 8.5. This can be maintained by adding a small amount of bicarbonate to the hatching water prior to adding the artemia cysts.
• Temperature should be maintained between 28 and 30 degrees celsius.

• Hydrate artemia cysts: This shortens hatching time and disinfects the cysts. This is done for one hour in a 5 – 15ppm chlorine solution with very strong aeration, making sure that all cysts get exposed to the chlorine solution.

1. Percent Hatch Rate (HR%)
   This is ascertained by first determining the Cyst Per Gram (CPG) count and then determining the Nauplii Per Gram count and dividing the two as NPG / CPG.